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automated hybridization station hs4800  (Tecan Systems)


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    Structured Review

    Tecan Systems automated hybridization station hs4800
    Automated Hybridization Station Hs4800, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/automated+hybridization+station/hs4800+hybridization+station/pm40664182-92-7-10
    Average 90 stars, based on 1 article reviews
    automated hybridization station hs4800 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Hybridization:

    Article Title: Functional genomics analysis of foliar condensed tannin and phenolic glycoside regulation in natural cottonwood hybrids.
    Article Snippet: .. Hybridization was carried out in an automated hybridization station (Tecan, Maennedorf, Switzerland) at 40 °C for 36 h (Sartor et al. 2004) using equal amounts of Cy3- and Cy5-labeled targets. .. The slides were scanned with a GenePix 4000B Scanner (Axon Instruments, Union City, CA) and the fluorescence intensity quantified with the GenePix Pro 5.1 image analysis software (Axon).

    Article Title: Inter-individual and inter-regional variability of breast milk antibody reactivity to bacterial lipopolysaccharides
    Article Snippet: .. All following processing steps were performed in an automated hybridization station (HS4800, Tecan, Männedorf, Switzerland), which allows to control temperature and duration of all incubation and washing steps. .. The incubations with 40 mg/mL Blotting-Grade Blocker (BioRad, Hercules, CA, USA) as blocking buffer, breast milk samples, and secondary antibody were followed by washing steps consisting of a rinse for 1 min with PBS-T followed by an agitation of the stationary solution for 3 min.

    Article Title:
    Article Snippet: All array experiments were carried out in two colours, Cy5 and Cy3, using the cRNA linear amplification and fluorescent labelling kit from Agilent Technologies. .. All arrays were processed in an automated hybridization station (Tecan), images were obtained with SCANARRAY EXPRESS® confocal laser scanner (Packard BioSystems), and image analysis was performed by QUANTARRAY® image analysis software (Packard Biosystems). ..

    Article Title: Array CGH demonstrates characteristic aberration signatures in human papillary thyroid carcinomas governed by RET/PTC.
    Article Snippet: For each experiment, 450ng of either female or male reference DNA (Promega, Mannheim, Germany) and 450ng tumour DNA were labelled with Cy3-dCTP and Cy5-dCTP (Perkin Elmer, Shelton, USA), respectively, in an overnight incubation at 37 1C using the BioPrime-Labelling Kit (Invitrogen, Karlsruhe, Germany). .. Test and reference DNAs were co-hybridized for 40h with 135mg cot-1 DNA (Invitrogen), washed and dried in an automated hybridization station (HS400, Tecan, Crailsheim, Germany). ..

    Article Title: Method for preventing the unfolding of a (poly)peptide and/or inducing the (re-)folding of a (poly)peptide
    Article Snippet: The protein microarrays were blocked in 2% (w/v) bovine serum albumin (BSA/TBST, 0.1% [v/v] Tween 20) at room temperature for 1 h. The antibody was added to 2% (w/v) BSA/TBST and was incubated in a humidified atmosphere for 16 h. Following three TBST washes and subsequent incubation with the secondary antibody (goat-anti-human-IgG-Cy3 (Dianova, Hamburg, Germany) at 0.002 mg/ml for 1 h at room temperature in 2% (w/v) BSA/TBST, the protein microarrays were washed three times in TBST. .. All antibody incubation steps were carried out in a volume of 200 μl in an automated hybridization station (Tecan HS 4800 Pro; Tecan, Crailsheim, Germany). .. Read out of the results was performed with a confocal microarray reader (ScanArray 4000, Perkin Elmer Life Science; San Jose, Calif.) using identical settings for all protein microarrays.

    Article Title: Clonal success of piliated penicillin nonsusceptible pneumococci
    Article Snippet: .. The hybridization was carried out at 42°C for 14 h in an automated hybridization station (TECAN HS400; Tecan Group, Maennedorf, Switzerland). ..

    Control:

    Article Title: Inter-individual and inter-regional variability of breast milk antibody reactivity to bacterial lipopolysaccharides
    Article Snippet: .. All following processing steps were performed in an automated hybridization station (HS4800, Tecan, Männedorf, Switzerland), which allows to control temperature and duration of all incubation and washing steps. .. The incubations with 40 mg/mL Blotting-Grade Blocker (BioRad, Hercules, CA, USA) as blocking buffer, breast milk samples, and secondary antibody were followed by washing steps consisting of a rinse for 1 min with PBS-T followed by an agitation of the stationary solution for 3 min.

    Incubation:

    Article Title: Inter-individual and inter-regional variability of breast milk antibody reactivity to bacterial lipopolysaccharides
    Article Snippet: .. All following processing steps were performed in an automated hybridization station (HS4800, Tecan, Männedorf, Switzerland), which allows to control temperature and duration of all incubation and washing steps. .. The incubations with 40 mg/mL Blotting-Grade Blocker (BioRad, Hercules, CA, USA) as blocking buffer, breast milk samples, and secondary antibody were followed by washing steps consisting of a rinse for 1 min with PBS-T followed by an agitation of the stationary solution for 3 min.

    Article Title: Method for preventing the unfolding of a (poly)peptide and/or inducing the (re-)folding of a (poly)peptide
    Article Snippet: The protein microarrays were blocked in 2% (w/v) bovine serum albumin (BSA/TBST, 0.1% [v/v] Tween 20) at room temperature for 1 h. The antibody was added to 2% (w/v) BSA/TBST and was incubated in a humidified atmosphere for 16 h. Following three TBST washes and subsequent incubation with the secondary antibody (goat-anti-human-IgG-Cy3 (Dianova, Hamburg, Germany) at 0.002 mg/ml for 1 h at room temperature in 2% (w/v) BSA/TBST, the protein microarrays were washed three times in TBST. .. All antibody incubation steps were carried out in a volume of 200 μl in an automated hybridization station (Tecan HS 4800 Pro; Tecan, Crailsheim, Germany). .. Read out of the results was performed with a confocal microarray reader (ScanArray 4000, Perkin Elmer Life Science; San Jose, Calif.) using identical settings for all protein microarrays.

    other:

    Article Title: Linking molecular biomarkers with higher level condition indicators to identify effects of copper exposures on the endangered delta smelt (Hypomesus transpacificus).
    Article Snippet: The delta smelt (Hypomesus transpacificus) is an endangered pelagic fish species endemic to the Sacramento–San Joaquin estuary (CA, USA), and considered an indicator of ecosystem health.. Copper is a contaminant of concern in Californian waterways that may affect the development and survival of this endangered species.. The experimental combination of molecular biomarkers with higher level effects may allow for interpretation of responses in a functional context that can be used to predict detrimental outcomes caused by exposure.

    Article Title: Linking mechanistic and behavioral responses to sublethal esfenvalerate exposure in the endangered delta smelt; Hypomesus transpacificus (Fam. Osmeridae)
    Article Snippet: Each experimental sample and control was combined with a reference pool cDNA prior to hybridization using an automated Tecan HS4800 hybridization station.

    Software:

    Article Title:
    Article Snippet: All array experiments were carried out in two colours, Cy5 and Cy3, using the cRNA linear amplification and fluorescent labelling kit from Agilent Technologies. .. All arrays were processed in an automated hybridization station (Tecan), images were obtained with SCANARRAY EXPRESS® confocal laser scanner (Packard BioSystems), and image analysis was performed by QUANTARRAY® image analysis software (Packard Biosystems). ..

    Automated:

    Article Title: Method for preventing the unfolding of a (poly)peptide and/or inducing the (re-)folding of a (poly)peptide
    Article Snippet: The protein microarrays were blocked in 2% (w/v) bovine serum albumin (BSA/TBST, 0.1% [v/v] Tween 20) at room temperature for 1 h. The antibody was added to 2% (w/v) BSA/TBST and was incubated in a humidified atmosphere for 16 h. Following three TBST washes and subsequent incubation with the secondary antibody (goat-anti-human-IgG-Cy3 (Dianova, Hamburg, Germany) at 0.002 mg/ml for 1 h at room temperature in 2% (w/v) BSA/TBST, the protein microarrays were washed three times in TBST. .. All antibody incubation steps were carried out in a volume of 200 μl in an automated hybridization station (Tecan HS 4800 Pro; Tecan, Crailsheim, Germany). .. Read out of the results was performed with a confocal microarray reader (ScanArray 4000, Perkin Elmer Life Science; San Jose, Calif.) using identical settings for all protein microarrays.



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